HisTrap EXCEL columns are prepacked with Ni Sepharose excel affinity media for capture and purification of histidine-tagged proteins secreted into eukaryotic cells.
• HiTrap columns are available in 1 ml and 5 ml bed volume • Each HiTrap™ column is equiped with one 1/16" male luer and one 1/16" female luer. It is possible to connect columns to each other for achieving columns of variable volume from 1 to 20 ml as required • Concentration or desalting samples • Purification of recombinant proteins • Purification of immunoglobulins
Acronyms and abbreviations: Q = Quaternary ammonium / DEAE = Diethylaminoethyle / CM = Carboxymethyle / S = Methylsulfonate / ANX = Diethylaminopropyle
HiTrap Capto™: designed to meet industrial requirements for the production of biological molecules.
HiTrap NHS: high-performance medium designed for easy coupling of ligands containing primary amines.
HiTrap Protein A and HiTrap Protein G: designed for preparative purification of monoclonal and polyclonal antibodies from ascites, serum, and cell culture supernatants.
HiTrap MabSelect™, MabSelect SuRe™ and MabSelect Xtra™: developed to meet the productivity and sanitisation requirements of industry to develop large-scale antibody purification procedures.
HiTrap IgM: designed for purification of monoclonal IgM.
HiTrap IgY: designed for purification of IgY.
HiTrap IXSelect: pour la purification du facteur de coagulation IX.
• Recombinant protein G coupled to Sepharose 4 Fast Flow (4 % agarose matrix) • Application: purification of monoclonal or polyclonal IgG antibodies • No specific albumin binding • Dynamic capacity biding: 18 mg human IgG / ml medium • pH working range: 3 to 9 • Storage: 2 to 8 °C
• nProtein A coupled to Sepharose 4 Fast Flow (4% agarose matrix) • Application: purification of monoclonal and polyclonal antibodies • Highly stable with minimal nonspecific adsorption • Binding capacity: 35 mg human IgG / ml of matrix • pH range: 3 - 9 • storage: 4 to 8 ° C